Journal: International journal of molecular sciences

Article Title: 2-Methoxyestradiol-3,17- O , O -bis-sulfamate (STX140) Inhibits Proliferation and Invasion via Senescence Pathway Induction in Human BRAFi-Resistant Melanoma Cells.

doi: 10.3390/ijms241411314

Figure Lengend Snippet: Figure 4. Anti-invasion activity of STX140 against human melanoma cells. (a) Reconstructed human skin with melanoma micrographs from inverted microscope (20× and 40×, respectively) dyed with hematoxylin/eosin (H&E); (b) Interleukin quantification in media from the 3D reconstructed human skin samples, STX140 (100 nM), results are expressed as the mean of three independent experiments (n = 3) ± S.D.; significance is indicated by *** p < 0.001.

Article Snippet: Interleukins IL-6 (QC193, R&D Systems®, Minneapolis, MN, EUA) and IL-8 (R&D Systems®, AF-208-NA) from samples were quantified by ELISA assay (R&D Systems®) and analyzed according to the instructions of the commercial kit manufacturers.

Techniques: Activity Assay, Inverted Microscopy

Journal: Theranostics

Article Title: Cancer-associated Fibroblast-derived IL-6 Promotes Head and Neck Cancer Progression via the Osteopontin-NF-kappa B Signaling Pathway

doi: 10.7150/thno.22182

Figure Lengend Snippet: Fibroblasts contribute to the up-regulation of OPN in HNC cells. (A) Immunohistochemical analysis of OPN protein expression in HNC tissues and matched adjacent normal tissues (Scale bar: 25 μm). Higher staining of OPN was observed in tumor cells of the edge of bulk tumors. (B) The mRNA and protein levels of OPN were determined in NFs, CAFs and cancer cells (derived from 4 HNC patients) using real-time PCR and western blotting. (C) OPN at the protein and mRNA level and in the supernatant was detected in 8 representative HNC cell lines and normal oral epithelial cells (titled normal) using western blotting, real-time PCR and ELISA. (D) The protein level, supernatant concentration and mRNA level of OPN were determined in CAL-27 and SCC-25 cells after co-culture with NFs. (E) The protein level, supernatant concentration and mRNA level of OPN were determined in NFs after co-culture with CAL-27 and SCC-25 cells. (* p <0.05; ** p <0.01; *** p <0.001; **** p <0.0001)

Article Snippet: The supernatant OPN and IL-6 concentrations and the plasma OPN and IL-6 levels in patients with HNC were assessed using the Human OPN ELISA kit (Boster, China) and human IL-6 ELISA kit (Boster, China).

Techniques: Immunohistochemical staining, Expressing, Staining, Derivative Assay, Real-time Polymerase Chain Reaction, Western Blot, Enzyme-linked Immunosorbent Assay, Concentration Assay, Co-Culture Assay

Journal: Theranostics

Article Title: Cancer-associated Fibroblast-derived IL-6 Promotes Head and Neck Cancer Progression via the Osteopontin-NF-kappa B Signaling Pathway

doi: 10.7150/thno.22182

Figure Lengend Snippet: Effects of stromal IL-6-induced OPN on promoting tumor growth and metastasis in vivo . (A) OPN overexpression in CAL-27 cells facilitated the xenograft tumor growth in nude mice. (B) Plasma OPN levels of the tumor-bearing mice from the normal group, CAL-27 group and CAL-27-OPN group. (C) Subcutaneous injection of OPN antibody (10 μg per tumor nodule) or IL-6 antibody (10 μg per tumor nodule) around the tumor partly inhibited NF-mediated tumor growth, and the combination of OPN antibody (5 μg per tumor nodule) and IL-6 antibody (5 μg per tumor nodule) exhibited a more powerful antitumor activity. (D) Plasma OPN levels of the tumor-bearing mice from the normal group, IgG treatment group, OPN antibody treatment group, IL-6 antibody treatment group and the combination group. (E) Overexpression of OPN in Rca-T cells promoted the formation and growth of metastatic nodules in nude mice (Scale bar, left: 5 mm; right: 100 μm). (F) Western blot analysis confirmed the exogenous expression of OPN in Rca-T cells, and plasma OPN levels of the mice involved in experimental metastasis were measured using ELISA. (G) Kaplan-Meier analyses of overall survival. (H, I and J) ROC curve analysis of the mRNA panel of OPN and IL-6 stratified by different groups in the validation set. ROC plots for the mRNA panel of OPN and IL-6 discriminated the five-year survival group from the death group (H), the TNM stage I group from the healthy controls (I), and the metastasis group from the non-metastasis group (J). AUC, area under the curve. (K) A proposed model illustrating the modulatory role of stromal IL-6-induced neoplastic OPN in controlling tumor growth and metastasis. (* p <0.05; ** p <0.01; *** p <0.001; **** p <0.0001)

Article Snippet: The supernatant OPN and IL-6 concentrations and the plasma OPN and IL-6 levels in patients with HNC were assessed using the Human OPN ELISA kit (Boster, China) and human IL-6 ELISA kit (Boster, China).

Techniques: In Vivo, Over Expression, Clinical Proteomics, Injection, Activity Assay, Western Blot, Expressing, Enzyme-linked Immunosorbent Assay, Biomarker Discovery

Journal: BMC Complementary and Alternative Medicine

Article Title: The synergistic antitumor effect of Huaier combined with 5-Florouracil in human cholangiocarcinoma cells

doi: 10.1186/s12906-019-2614-5

Figure Lengend Snippet: Huaier synergized with 5-FU promoting the inhibition of both constitutive and Inducible STAT3 phosphorylation by IL-6. The total STAT3 and p-STAT proteins expression in cytoplasm ( a and b ) and nucleus ( c and d ) were detected using Western blot after 48 h treatment with Huaier and 5-FU alone or in combination. In the meantime, the effect of combination therapy on p-STAT3 Induced by IL-6 were also checked. Relative optical density of proteins were analyzed by Image J. All results are expressed as mean ± SD of three independent experiments. * P < 0.05, ** P < 0.01, *** P < 0.001, compared with control group, while # P < 0.05, ## P < 0.01, ### P < 0.001, compared with combination group

Article Snippet: IL-6/ Interleukin-6 was provided by Sino Biological Inc. (Beijing, China).

Techniques: Inhibition, Expressing, Western Blot